Volume 4, Issue 4, July 2018, Page: 63-67
A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis
Zexian Chen, Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Yongle Chen, Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Xiaowen He, Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Ping Lan, Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Received: Sep. 6, 2018;       Accepted: Sep. 20, 2018;       Published: Oct. 12, 2018
DOI: 10.11648/j.ijcems.20180404.12      View  242      Downloads  21
Abstract
Inflammatory bowel disease, including Crohn’s disease and ulcerative colitis, is one kind of chronic, relapsing inflammatory disorder of the gastrointestinal tract and has the potential of tumorigenesis. However, no stable and mature cell line is available for relative basic research, so animal model is essential. In this study, a mice model was built, which mimics the dynamic disease course of inflammatory bowel disease associated tumorigenesis with injection of azoxymethane and sequent cycles of drinking water with dextran sulfate sodium. All the mice survived till the end of the modeling procedure. Compared with the mice in the control group, the mice in the modeling group experienced obvious bloody diarrhea and body weight loss (P<0.001). The colorectum was significantly swollen and shorter (P<0.001) while the spleen was significantly bigger (P<0.001) in mice in the modeling group compared with the mice in the control group. All the mice in the modeling group developed tumors in the colorectum, with an average tumor number of 6.5 and an average tumor size of 10.25 mm3. Pathological evaluation by hematoxylin and eosin staining confirmed the tumors as adenomas with high-grade dysplasia. In conclusion, this model is inducible and stable which would be very useful in the research in this field.
Keywords
Inflammatory Bowel Disease, Tumorigenesis, Mice Model
To cite this article
Zexian Chen, Yongle Chen, Xiaowen He, Ping Lan, A Mouse Model for Inflammatory Bowel Disease Associated Tumorigenesis, International Journal of Clinical and Experimental Medical Sciences. Vol. 4, No. 4, 2018, pp. 63-67. doi: 10.11648/j.ijcems.20180404.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Endo K, Shiga H, Kinouchi Y, Shimosegawa T. Inflammatory bowel disease: IBD. Rinsho Byori, 2009, 57(6): 527-532.
[2]
Soderlund S, Brandt L, Lapidus A, Karlen P, Brostrom O, Lofberg R, et al. Decreasing time-trends of colorectal cancer in a large cohort of patients with inflammatory bowel disease. Gastroenterology, 2009, 136(5): 1561-1567, 1818-1819.
[3]
Adami H O, Bretthauer M, Emilsson L, Hernan M A, Kalager M, Ludvigsson J F, et al. The continuing uncertainty about cancer risk in inflammatory bowel disease. Gut, 2016, 65(6): 889-893.
[4]
Itzkowitz S H, Harpaz N. Diagnosis and management of dysplasia in patients with inflammatory bowel diseases. Gastroenterology, 2004, 126(6): 1634-1648.
[5]
Vagefi P A, Longo W E. Colorectal cancer in patients with inflammatory bowel disease. Clin Colorectal Cancer, 2005, 4(5): 313-319.
[6]
Herszenyi L, Miheller P, Tulassay Z. Carcinogenesis in inflammatory bowel disease. Dig Dis, 2007, 25(3): 267-269.
[7]
Eaden J A, Abrams K R, Mayberry J F. The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut, 2001, 48(4): 526-535.
[8]
Jess T, Gamborg M, Matzen P, Munkholm P, Sorensen T I. Increased risk of intestinal cancer in Crohn's disease: a meta-analysis of population-based cohort studies. Am J Gastroenterol, 2005, 100(12): 2724-2729.
[9]
Lakatos L, Mester G, Erdelyi Z, David G, Pandur T, Balogh M, et al. Risk factors for ulcerative colitis-associated colorectal cancer in a Hungarian cohort of patients with ulcerative colitis: results of a population-based study. Inflamm Bowel Dis, 2006, 12(3): 205-211.
[10]
Loftus E J. Epidemiology and risk factors for colorectal dysplasia and cancer in ulcerative colitis. Gastroenterol Clin North Am, 2006, 35(3): 517-531.
[11]
Grivennikov S I, Greten F R, Karin M. Immunity, inflammation, and cancer. Cell, 2010, 140(6): 883-899.
[12]
Fitzpatrick F A. Inflammation, carcinogenesis and cancer. Int Immunopharmacol, 2001, 1(9-10): 1651-1667.
[13]
Ullman T A, Itzkowitz S H. Intestinal inflammation and cancer. Gastroenterology, 2011, 140(6): 1807-1816.
[14]
Baker A M, Cross W, Curtius K, Al B I, Choi C R, Davis H L, et al. Evolutionary history of human colitis-associated colorectal cancer. Gut, 2018.
[15]
Liu Y, Lu Y, Zhou M, Zhang C, Li X. Construction of colorectal cancer cell line stably expressing mir-101 and identification of the target gene of mir-101. Nan Fang Yi Ke Da Xue Xue Bao, 2014, 34(7): 928-933.
[16]
Mathieu A A, Ohl-Seguy E, Dubois M L, Jean D, Jones C, Boudreau F, et al. Subcellular proteomics analysis of different stages of colorectal cancer cell lines. Proteomics, 2016, 16(23): 3009-3018.
[17]
Boivin G P, Washington K, Yang K, Ward J M, Pretlow T P, Russell R, et al. Pathology of mouse models of intestinal cancer: consensus report and recommendations. Gastroenterology, 2003, 124(3): 762-777.
[18]
Day C P, Merlino G, Van Dyke T. Preclinical mouse cancer models: a maze of opportunities and challenges. Cell, 2015, 163(1): 39-53.
[19]
Dotti I, Salas A. Potential Use of Human Stem Cell-Derived Intestinal Organoids to Study Inflammatory Bowel Diseases. Inflamm Bowel Dis, 2018.
[20]
Manicassamy S, Manoharan I. Mouse models of acute and chronic colitis. Methods Mol Biol, 2014, 1194: 437-448.
[21]
Ostanin D V, Bao J, Koboziev I, Gray L, Robinson-Jackson S A, Kosloski-Davidson M, et al. T cell transfer model of chronic colitis: concepts, considerations, and tricks of the trade. Am J Physiol Gastrointest Liver Physiol, 2009, 296(2): G135-G146.
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